Fatemeh Saadat; Seyed Hadi Razavi; Houshang Alizadeh
Abstract
Introduction: Oil plants store energy in the form of neutral lipids in the organelles called oil bodies. These organelles save triacylglycerol until seed germination. In recent years, the oil bodies have been considered as an oil/water emulsion in the pharmaceutical, food, and cosmetic industries. These ...
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Introduction: Oil plants store energy in the form of neutral lipids in the organelles called oil bodies. These organelles save triacylglycerol until seed germination. In recent years, the oil bodies have been considered as an oil/water emulsion in the pharmaceutical, food, and cosmetic industries. These organelles are also effective tool for purifying, stabilizing and delivery of biotechnology products. Aqueous extraction processing (AEP) is the most common method for oil body extraction. Despite all advantages compared to organic solvent extraction, the yield of AEP still needs to be optimized. Therefore, this study surveys the efficacy of two solvents, phosphate buffer and distilled water in the oil bodies' extraction from rapeseed. Materials and methods: Brassica napus L. seeds were obtained from seed and plant improvement institute, Iran. To compare the efficacy of solvents, 0.1 M phosphate buffer (pH 7.5) and distilled water were used for extraction. The ground rapeseed was suspended in the buffers in a ratio of 1:10 (w/v) and stirred for 12 hours at room temperature. This step was repeated three times. Then, the extract was centrifuged at 10,000 g for 15 minutes at 4 ° C. The floating layer was carefully removed and dissolved again in the initial solvent and the pH was adjusted to 8.5 to precipitate the deflated proteins. Finally, the cream layer was retrieved using centrifuges and one-tenth of the initial buffer volume was applied to the 9 M urea buffer (pH 7.5) for 10 minutes to separate non-specific proteins from oil bodies. The purified oil-bodies were monitored under light microscopy. Results and discussion: According to the microscopic and macroscopic results, the stability of oil particles and efficiency of extraction would be higher by phosphate buffer due to maintaining a constant alkaline pH during the extraction. Moreover, the presence of different salts in the phosphate buffer increases the purification yield up to twice times as a result of providing osmotic pressure and increasing solubility of membrane proteins. These results emphasize the importance of membrane proteins on the formation and stabilization of oil bodies.
Fatemeh Raiesi; Mohammad Hojjatoleslami; Seyed Hadi Razavi; Mahdi Bahman; Mohammad Ali Shariaty
Abstract
In this study, three varieties of rice including Tarom, Anbarbo and Hashemi were selected and their heavy metals Content were analyzed. Subcritical water was used to provide rice bran extract at temperatures 100 and 120 ° C. In order to provide grape juice treatments, we used 5, 10, 15 and 20 % of extract ...
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In this study, three varieties of rice including Tarom, Anbarbo and Hashemi were selected and their heavy metals Content were analyzed. Subcritical water was used to provide rice bran extract at temperatures 100 and 120 ° C. In order to provide grape juice treatments, we used 5, 10, 15 and 20 % of extract in the formulation of red grape drink. samples which had packed and pasteurized, kept in the refrigerator for three months, then Rheological measurements were carried out by using Brookfield rheometer at 5°C .Physical and chemical characteristic evaluated by using SPSS software and one-way ANOVA and Duncan test >P) 0.05). Increasing temperature results in increasing rice bran extraction in subcritical water extraction method. Investigation of rheological properties showed that increasing in amount of rice bran extract cause to decrease consistency coefficient and increase flow index. Consistency coefficient of treatments during storage indicated that consistency coefficients were increased for all treatment
Zahra Emam-Djomeh; Manuchehr Hamedi; Seyed Hadi Razavi; Mohammad Taghi Golmakani
Abstract
In this study the antimicrobial effects of essential oils from dill and coriander seeds on
staphylococcus aureus, Escherichia coli O157:H7 and salmonella typhimuruim were investigated and
the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of each
essential oil ...
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In this study the antimicrobial effects of essential oils from dill and coriander seeds on
staphylococcus aureus, Escherichia coli O157:H7 and salmonella typhimuruim were investigated and
the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of each
essential oil were determinate. For this purpose 5 concentration of each essential oils (125, 250, 500,
1000, 2000 and 4000 ppm) were chosen. For microbial count, Broth Dilution Test with Mueller
Hinton Agar and Broth were used. Results showed that Staphylococcus aureus had more susceptibility
and Salmonella typhimuruim was the resistant one. Our results also showed that essential oil from
coriander seed had more antimicrobial effect on the gram-negative bacteria. The essential oil from
coriander seed had MIC and MBC equal to 1000ppm and the essential oil from dill seed had MIC
equal to 500ppm and MBC equal to 1000 ppm against Staphylococcus aureus.
Key word: Broth Dilution Test, Staphylococcus aureus, Escherichia coli O157:H7, Salmonella
typhimuruim, essential oils, dill seed, coriander seed
Seyed Hadi Razavi; Keramatoallah Rezaee tireh shabankareh; Ivan Mark
Abstract
Abstract
In this study, the extraction of carotenoids from a new strain of yeast (Sporobolomyces ruberrimus H110) by different chemical and physical methods was investigated. For chemical pretreatment methods, HCl at two different concentrations (0.2 and 0.5 N) and two different temperatures (55 ...
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Abstract
In this study, the extraction of carotenoids from a new strain of yeast (Sporobolomyces ruberrimus H110) by different chemical and physical methods was investigated. For chemical pretreatment methods, HCl at two different concentrations (0.2 and 0.5 N) and two different temperatures (55 and 75C), and DMSO solvent (also at two different temperatures, 55 and 75C) were used. Sonication and bead beater were applied as physical methods. Results showed that the highest pigment extraction level (~100%) obtained by using bead beater. The second best method was the pretreatment by DMSO. Compared to chemical solvents used in this study, the use of a bead beater for the extraction of pigments is the best approach to avoid the toxicity risk(s) involved in the extraction process by DMSO.
Key Words: Extraction, Pigment, Carotenoids, Sporobolomyces ruberrimus, DMSO, Bead
Beater
